Bead-beating assay during synovial fluid DNA extraction improves real-time PCR accuracy for periprosthetic joint infection.
editorial · Level V
Where this comes from
- Record sourced from PubMed, PMID 38741241.
- Also identified by DOI 10.1002/jor.25871.
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Abstract
Polymerase chain reaction (PCR)-based genetic diagnosis is a rapid and sensitive method to diagnose periprosthetic joint infection (PJI). DNA extraction using bead beating is an effective method for collecting bacterial genes in Gram-positive bacteria. We compared the detection accuracy between the conventional and bead-beating DNA extraction assay. The detection rate improved from 86.7% using the conventional method to 95.6% using the bead-beating. Our results suggest that bead-beating during DNA extraction can improve the accuracy of PCR-based genetic diagnosis of PJI.
Medical subject headings
- Synovial Fluid
- Prosthesis-Related Infections
- Real-Time Polymerase Chain Reaction
- DNA, Bacterial