Automated dynamic inlet microfluidics system: 3D printer adaptation for cost-effective, low volume, on-demand multi-analyte droplet generator.
basic_science · Level V
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- Record sourced from PubMed, PMID 38745471.
- Also identified by DOI 10.1039/d4lc00075g.
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Abstract
The paper demonstrates an adaptation of a 3D printer (Prusa Mini+) with novel modules to develop a droplet generation system that generates combinatorial droplets from a standard 96 well plate. The calibration methodology developed would allow any fused deposition modeling (FDM) printer to generate monodisperse droplets (coefficient of variance (CV%) < 5%) from well plates or vials of any geometry. The system maintains precision across various volumes while maintaining a C.V. range of 0.81% to 3.61%, with an increased precision for larger volumes. The cost of the system developed is 70% less than commercially available droplet generation packages. Successful droplet library storage is accomplished <i>via</i> 3D printed cartridge connectors. The implemented system has been calibrated for Tygon® and PTFE at different velocities and volumetric configurations.