Interferometric Biosensor for High Sensitive Label-Free Recording of HiPS Cardiomyocytes Contraction <i>in Vitro</i>.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38776267.
- Also identified by DOI 10.1021/acs.nanolett.3c04291 and PMC identifier 11157657.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Heart disease remains a leading cause of global mortality, underscoring the need for advanced technologies to study cardiovascular diseases and develop effective treatments. We introduce an innovative interferometric biosensor for high-sensitivity and label-free recording of human induced pluripotent stem cell (hiPSC) cardiomyocyte contraction <i>in vitro</i>. Using an optical cavity, our device captures interference patterns caused by the contraction-induced displacement of a thin flexible membrane. First, we demonstrate the capability to quantify spontaneous contractions and discriminate between contraction and relaxation phases. We calculate a contraction-induced vertical membrane displacement close to 40 nm, which implies a traction stress of 34 ± 4 mN/mm<sup>2</sup>. Finally, we investigate the effects of a drug compound on contractility amplitude, revealing a significant reduction in contractile forces. The label-free and high-throughput nature of our biosensor may enhance drug screening processes and drug development for cardiac treatments. Our interferometric biosensor offers a novel approach for noninvasive and real-time assessment of cardiomyocyte contraction.
Medical subject headings
- Myocytes, Cardiac
- Induced Pluripotent Stem Cells
- Biosensing Techniques
- Interferometry
- Myocardial Contraction