MUSTANG: Multi-sample spatial transcriptomics data analysis with cross-sample transcriptional similarity guidance.

Niyakan, Seyednami; Sheng, Jianting; Cao, Yuliang; Zhang, Xiang; Xu, Zhan; Wu, Ling; Wong, Stephen T C; Qian, Xiaoning · Patterns (N Y) · 2024

basic_science · Level V

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Abstract

Spatially resolved transcriptomics has revolutionized genome-scale transcriptomic profiling by providing high-resolution characterization of transcriptional patterns. Here, we present our spatial transcriptomics analysis framework, MUSTANG (MUlti-sample Spatial Transcriptomics data ANalysis with cross-sample transcriptional similarity Guidance), which is capable of performing multi-sample spatial transcriptomics spot cellular deconvolution by allowing both cross-sample expression-based similarity information sharing as well as spatial correlation in gene expression patterns within samples. Experiments on a semi-synthetic spatial transcriptomics dataset and three real-world spatial transcriptomics datasets demonstrate the effectiveness of MUSTANG in revealing biological insights inherent in the cellular characterization of tissue samples under study.