Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38806736.
- Also identified by DOI 10.1038/s41587-024-02266-4.
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Abstract
Therapeutic prime editing of hematopoietic stem and progenitor cells (HSPCs) holds great potential to remedy blood disorders. Quiescent cells have low nucleotide levels and resist retroviral infection, and it is possible that nucleotide metabolism could limit reverse transcription-mediated prime editing in HSPCs. We demonstrate that deoxynucleoside supplementation and Vpx-mediated degradation of SAMHD1 improve prime editing efficiency in HSPCs, especially when coupled with editing approaches that evade mismatch repair.
Medical subject headings
- Hematopoietic Stem Cells
- Gene Editing
- Nucleotides