High-throughput volumetric mapping of synaptic transmission.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38898094.
- Also identified by DOI 10.1038/s41592-024-02309-3.
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Abstract
Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.
Medical subject headings
- Synaptic Transmission
- Synapses