Rapid bacterial evaluation beyond the colony forming unit in osteomyelitis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38910553.
- Also identified by DOI 10.7554/eLife.93698 and PMC identifier 11196105.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Examination of bacteria/host cell interactions is important for understanding the aetiology of many infectious diseases. The colony forming unit (CFU) has been the standard for quantifying bacterial burden for the past century, however, this suffers from low sensitivity and is dependent on bacterial culturability in vitro. Our data demonstrate the discrepancy between the CFU and bacterial genome copy number in an osteomyelitis-relevant co-culture system and we confirm diagnosis and quantify bacterial load in clinical bone specimens. This study provides an improved workflow for the quantification of bacterial burden in such cases.
Medical subject headings
- Osteomyelitis