Palladium/Platinum/Ruthenium Trimetallic Dendritic Nanozymes Exhibiting Enhanced Peroxidase-like Activity for Signal Amplification of Lateral Flow Immunoassays.
basic_science · Level V
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- Record sourced from PubMed, PMID 38935481.
- Also identified by DOI 10.1021/acs.nanolett.4c01568.
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Abstract
Developing ultrasensitive lateral flow immunoassays (LFIAs) has garnered significant attention in the field of point-of-care testing. In this study, a trimetallic dendritic nanozyme (Pd@Pt-Ru) was synthesized through Ru deposition on a Pd@Pt core and utilized to enhancing the sensitivity of LFIAs. Pd@Pt-Ru exhibited a <i>K</i><sub>m</sub> value of 5.23 mM for detecting H<sub>2</sub>O<sub>2</sub>, which indicates an H<sub>2</sub>O<sub>2</sub> affinity comparable with that of horseradish peroxidase. The Ru surface layer reduces the activation energy barrier, which increases the maximum reaction rate. As a proof of concept, the proposed Pd@Pt-Ru nanozyme was incorporated into LFIAs (A-Pd@Pt-Ru-LFIAs) for detecting human chorionic gonadotropin (hCG). Compared with conventional gold nanoparticle (AuNP)-LFIAs, A-Pd@Pt-Ru-LFIAs demonstrated 250-fold increased sensitivity, thereby enabling a visible detection limit as low as 0.1 IU/L. True positive and negative rates both reached 100%, which renders the proposed Pd@Pt-Ru nanozyme suitable for detecting hCG in clinical samples.
Medical subject headings
- Palladium
- Platinum
- Ruthenium
- Chorionic Gonadotropin
- Metal Nanoparticles
- Hydrogen Peroxide
- Limit of Detection