Skeletal abnormalities, pediatric-onset severe osteoporosis, and multiple fragility fractures in a patient with a novel <i>CTNNB1 de novo</i> variant.

Lesnyak, Olga; Marini, Francesca; Sokolnikova, Polina; Sorokina, Margarita; Sukhareva, Kseniya; Artamonova, Irina; Kenis, Vladimir; Tkach, Olga et al. · Bone Rep · 2024

case_report · Level V

Where this comes from

Abstract

We report a case of a patient with a <i>de novo</i> germline heterozygous truncating variant of <i>CTNNB1</i> gene (c.2172del, p.Tyr724Ter) causing neurodevelopmental disorder with spastic diplegia and visual defects syndrome (NEDSDV) associated with a new clinical feature - severe pediatric-onset osteoporosis and multiple fractures. A functional effect of the identified variant was demonstrated using adipose-tissue derived primary mesenchymal stem cells, where we detected the alteration of <i>CTNNB1</i>mRNA and β-catenin protein levels using real-time PCR and Western blot analysis.