Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38969722.
- Also identified by DOI 10.1038/s41592-024-02344-0 and PMC identifier 11399097.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.
Medical subject headings
- X-Ray Diffraction