Rotational and translational diffusion of biomolecules in complex liquids and HeLa cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 38995242.
- Also identified by DOI 10.1039/d4sm00422a.
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Abstract
Diffusive motion accompanies many physical and biological processes. The Stokes-Sutherland-Einstein relation for the translational diffusion coefficient, <i>D</i><sub>T</sub>, agrees with experiments done in simple fluids but fails for complex fluids. Moreover, the interdependence between <i>D</i><sub>T</sub> and rotational diffusion coefficient, <i>D</i><sub>R</sub>, also deviates in complex fluids from the classical relation of <i>D</i><sub>T</sub>/<i>D</i><sub>R</sub> = 4<i>r</i><sup>2</sup>/3 known in simple fluids. Makuch <i>et al. Soft Matter</i>, 2020, <b>16</b>, 114-124 presented a generalization of the classical translational and rotational diffusion theory for complex fluids. In this work, we empirically verify this model based on simultaneous translational and rotational diffusion measurements. We use fluorescently stained cowpea chlorotic mottle virus (CCMV) particles as monodisperse probes and aqueous polyethylene glycol (PEG) solutions as a model complex fluid. The theory and experimental data obtained from fluorescence correlation spectroscopy (FCS) measurements agreed. Finally, we used the same model and analyzed the diffusion of Yo-Pro-1 stained large ribosomal subunits (LSU) in the cytoplasm and nucleus of living HeLa cells.
Medical subject headings
- Polyethylene Glycols