Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39169056.
- Also identified by DOI 10.1038/s41467-024-51654-6 and PMC identifier 11339384.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.
Medical subject headings
- Bacillus subtilis
- Spores, Bacterial
- Protein Biosynthesis
- Ribosomes
- Bacterial Proteins
- Gene Expression Regulation, Bacterial
- Ribosomal Proteins