Simultaneous multicolor fluorescence imaging using PSF splitting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39242832.
- Also identified by DOI 10.1038/s41592-024-02383-7 and PMC identifier 12558347.
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Abstract
We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the 'Circulator', an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.
Medical subject headings
- Microscopy, Fluorescence