OsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39288070.
- Also identified by DOI 10.7554/eLife.96553 and PMC identifier 11407766.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
As a master regulator of seed development, Leafy Cotyledon 1 (LEC1) promotes chlorophyll (Chl) biosynthesis in <i>Arabidopsis</i>, but the mechanism underlying this remains poorly understood. Here, we found that loss of function of <i>OsNF-YB7</i>, a <i>LEC1</i> homolog of rice, leads to chlorophyllous embryo, indicating that <i>OsNF-YB7</i> plays an opposite role in Chl biosynthesis in rice compared with that in <i>Arabidopsis</i>. OsNF-YB7 regulates the expression of a group of genes responsible for Chl biosynthesis and photosynthesis by directly binding to their promoters. In addition, OsNF-YB7 interacts with Golden 2-Like 1 (OsGLK1) to inhibit the transactivation activity of OsGLK1, a key regulator of Chl biosynthesis. Moreover, OsNF-YB7 can directly repress <i>OsGLK1</i> expression by recognizing its promoter in vivo, indicating the involvement of OsNF-YB7 in multiple regulatory layers of Chl biosynthesis in rice embryo. We propose that OsNF-YB7 functions as a transcriptional repressor to regulate Chl biosynthesis in rice embryo.
Medical subject headings
- Oryza
- Chlorophyll
- Gene Expression Regulation, Plant
- Plant Proteins