Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39298669.
- Also identified by DOI 10.1021/acs.nanolett.4c02452.
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Abstract
Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of ∼100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
Medical subject headings
- Microscopy, Fluorescence
- Fluorescent Dyes
- Microscopy, Confocal