Evaluation of biocontrol efficacy of rhizosphere Pseudomonas aeruginosa for management of Phytophthora capsici of pepper.

Abstract

A significant population of biocontrol microorganisms resides in the rhizosphere of plants, which can be utilized for plant disease control. To explore the potential of rhizosphere soil microorganisms as biocontrol agents against pepper blight, a bacterial strain Pa608 was screened from rhizosphere soil of pepper and identified as Pseudomonas aeruginosa through morphological characteristics and 16S rRNA sequences. The result showed that the strain Pa608 demonstrated antagonistic activity against Phytophthora capsici, effectively suppressing mycelial growth. The potted experiment showed a high control efficacy of 88.0%. Remarkably, the strain Pa608 also reduced the disease index of pepper blight in the field, resulting in control efficiencies of 74.9%. Moreover, the strain Pa608 also enhanced pepper plant height and yield. GC-MS analysis revealed the production of numerous secondary metabolites by the strain Pa608, with α-pinene displaying potent anti-oomycete activity by inhibiting P. capsici growth. In conclusion, P. aeruginosa Pa608 exhibited high biocontrol activity against P. capsici and can be utilized for the management of P. capsici in pepper cultivation.