Increasing intracellular dNTP levels improves prime editing efficiency.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39322763.
- Also identified by DOI 10.1038/s41587-024-02405-x and PMC identifier 12092096.
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Abstract
In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.
Medical subject headings
- Gene Editing
- Deoxyribonucleotides
- RNA-Directed DNA Polymerase