The role of forkhead box M1-methionine adenosyltransferase 2 A/2B axis in liver inflammation and fibrosis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39333125.
- Also identified by DOI 10.1038/s41467-024-52527-8 and PMC identifier 11436801.
- Licence recorded as CC BY-NC-ND.
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Abstract
Methionine adenosyltransferase 2 A (MAT2A) and MAT2B are essential for hepatic stellate cells (HSCs) activation. Forkhead box M1 (FOXM1) transgenic mice develop liver inflammation and fibrosis. Here we examine if they crosstalk in male mice. We found FOXM1/MAT2A/2B are upregulated after bile duct ligation (BDL) and carbon tetrachloride (CCl<sub>4</sub>) treatment in hepatocytes, HSCs and Kupffer cells (KCs). FDI-6, a FOXM1 inhibitor, attenuates the development and reverses the progression of CCl<sub>4</sub>-induced fibrosis while lowering the expression of FOXM1/MAT2A/2B, which exert reciprocal positive regulation on each other transcriptionally. Knocking down any of them lowers HSCs and KCs activation. Deletion of FOXM1 in hepatocytes, HSCs, and KCs protects from BDL-mediated inflammation and fibrosis comparably. Interestingly, HSCs from Foxm1<sup>Hep-/-</sup>, hepatocytes from Foxm1<sup>HSC-/-</sup>, and HSCs and hepatocytes from Foxm1<sup>KC-/-</sup> have lower FOXM1/MAT2A/2B after BDL. This may be partly due to transfer of extracellular vesicles between different cell types. Altogether, FOXM1/MAT2A/MAT2B axis drives liver inflammation and fibrosis.
Medical subject headings
- Methionine Adenosyltransferase
- Forkhead Box Protein M1
- Liver Cirrhosis
- Hepatocytes
- Kupffer Cells
- Carbon Tetrachloride
- Hepatic Stellate Cells