Cooperative chemoenzymatic and biocatalytic cascades to access chiral sulfur compounds bearing C(sp<sup>3</sup>)-S stereocentres.

Zhao, Fei; Mattana, Ariane; Alam, Ruqaiya; Montgomery, Sarah L; Pandya, Akash; Manetti, Fabrizio; Dominguez, Beatriz; Castagnolo, Daniele · Nat Commun · 2024

basic_science · Level V

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Abstract

Biocatalysis has been widely employed for the generation of carbon-carbon/heteroatom stereocentres, yet its application in chiral C(sp<sup>3</sup>)-S bond construction is rare and limited to enzymatic kinetic resolutions. Herein, we describe the enantioselective construction of chiral C(sp<sup>3</sup>)-S bonds through ene-reductase biocatalyzed conjugate reduction of prochiral vinyl sulfides. A series of cooperative sequential/concurrent chemoenzymatic and biocatalytic cascades have been developed to access a broad range of chiral sulfides, including valuable β-hydroxysulfides bearing two adjacent C(sp<sup>3</sup>)-S and C(sp<sup>3</sup>)-O stereocentres, in a stereoconvergent manner with good to excellent yields (up to 96%) and enantioselectivities (up to >99% ee). Notably, this biocatalytic strategy allows to overcome the long-standing shortcomings of catalyst poisoning and C(sp<sup>2</sup>)/C(sp<sup>3</sup>)-S bond cleavage faced in transition-metal-catalyzed hydrogenation of vinyl sulfides. Finally, the potential of this methodology is also exemplified by its broader application in the stereoconvergent assembly of chiral C(sp<sup>3</sup>)-N/O/Se bonds with good to excellent enantioselctivities.