Application of stretchable strain sensors and an inertial measurement unit for simulative tension analysis of the calcaneofibular ligament in formalin-fixed cadavers.

Abstract

The calcaneofibular ligament, a component of the lateral ligament complex of the ankle joint, plays an essential role in ankle-joint stability. To understand the mechanism of sprain-induced calcaneofibular ligament injury, the effect of ankle positions on calcaneofibular ligament tension needs to be ascertained. We propose a convenient method that combines stretchable strain sensors and an inertial measurement unit for simulative tension analysis of the calcaneofibular ligament in formalin-fixed cadavers. The stretchable strain sensor was pre-stretched approximately 1.3 times and, then set along the direction of the calcaneofibular ligament; a capacitance value from the sensor was used as a parameter to reflect the tension generated. Accurate three-axial inertial measurement unit-based monitoring of joint angles was undertaken for ten cadaveric ankles in measurements at 10° intervals from 30° plantarflexion to 20° dorsiflexion, followed by the investigation of additional effects with 10° inversion and eversion. Two-way repeated-measures ANOVA revealed a significant interactive effect for plantar/dorsiflexion × inversion/eversion and main effects for plantar/dorsiflexion and inversion/eversion. Post hoc pairwise analysis confirmed that 20° dorsiflexion or 10° inversion induces tension, whereas 10° eversion causes relaxation. Moreover, a promotional interactive effect by 20° dorsiflexion and 10° inversion and an offsetting effect by 10° eversion to 20° dorsiflexion were revealed. The measured values showed high levels of reliability and reproducibility (intraclass correlation coefficient [1,1] = 0.89). These results appropriately demonstrate the tensile action of calcaneofibular ligament. The novel approach investigated herein potentially opens new avenues for precise ligament-function evaluation.