Model-directed generation of artificial CRISPR-Cas13a guide RNA sequences improves nucleic acid detection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39394482.
- Also identified by DOI 10.1038/s41587-024-02422-w and PMC identifier 12439118.
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Abstract
CRISPR guide RNA sequences deriving exactly from natural sequences may not perform optimally in every application. Here we implement and evaluate algorithms for designing maximally fit, artificial CRISPR-Cas13a guides with multiple mismatches to natural sequences that are tailored for diagnostic applications. These guides offer more sensitive detection of diverse pathogens and discrimination of pathogen variants compared with guides derived directly from natural sequences and illuminate design principles that broaden Cas13a targeting.
Medical subject headings
- CRISPR-Cas Systems
- RNA, Guide, CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats