Building pangenome graphs.

Garrison, Erik; Guarracino, Andrea; Heumos, Simon; Villani, Flavia; Bao, Zhigui; Tattini, Lorenzo; Hagmann, Jörg; Vorbrugg, Sebastian et al. · Nat Methods · 2024

basic_science · Level V

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Abstract

Pangenome graphs can represent all variation between multiple reference genomes, but current approaches to build them exclude complex sequences or are based upon a single reference. In response, we developed the PanGenome Graph Builder, a pipeline for constructing pangenome graphs without bias or exclusion. The PanGenome Graph Builder uses all-to-all alignments to build a variation graph in which we can identify variation, measure conservation, detect recombination events and infer phylogenetic relationships.

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