DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity.

Abstract

DYRK1A, a ubiquitously expressed kinase<i>,</i> is linked to the dominant intellectual developmental disorder, microcephaly, and Down syndrome in humans. It regulates numerous cellular processes such as cell cycle, vesicle trafficking, and microtubule assembly. DYRK1A is a critical regulator of organ growth; however, how it regulates organ growth is not fully understood. Here, we show that the knockdown of <i>DYRK1A</i> in mammalian cells results in reduced cell size, which depends on mTORC1. Using proteomic approaches, we found that DYRK1A interacts with the tuberous sclerosis complex (TSC) proteins, namely TSC1 and TSC2, which negatively regulate mTORC1 activation. Furthermore, we show that DYRK1A phosphorylates TSC2 at T1462, a modification known to inhibit TSC activity and promote mTORC1 activity. We also found that the reduced cell growth upon knockdown of DYRK1A can be rescued by overexpression of RHEB, an activator of mTORC1. Our findings suggest that DYRK1A inhibits TSC complex activity through inhibitory phosphorylation on TSC2, thereby promoting mTORC1 activity. Furthermore, using the <i>Drosophila</i> neuromuscular junction as a model, we show that the <i>mnb,</i> the fly homologs of <i>DYRK1A</i>, is rescued by RHEB overexpression, suggesting a conserved role of <i>DYRK1A</i> in TORC1 regulation.