A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39551783.
- Also identified by DOI 10.1038/s41467-024-54210-4 and PMC identifier 11570635.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Insecticide resistance (IR) poses a significant global challenge to public health and welfare. Here, we develop a locally-acting unitary self-eliminating allelic-drive system, inserted into the Drosophila melanogaster yellow (y) locus. The drive cassette encodes both Cas9 and a single gRNA to bias inheritance of the favored wild-type (1014 L) allele over the IR (1014 F) variant of the voltage-gated sodium ion channel (vgsc) target locus. When enduring a fitness cost, this transiently-acting drive can increase the frequency of the wild-type allele to 100%, depending on its seeding ratio, before being eliminated from the population. However, in a fitness-neutral "hover" mode, the drive maintains a constant frequency in the population, completely converting IR alleles to wild-type, even at low initial seeding ratios.
Medical subject headings
- Insecticide Resistance
- Alleles
- Drosophila melanogaster
- Transgenes