Tracking Insulin- and Glucagon-Expressing Cells In Vitro and In Vivo Using a Double-Reporter Human Embryonic Stem Cell Line.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39561351.
- Also identified by DOI 10.2337/db24-0756 and PMC identifier 11755683.
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Abstract
Differentiation protocols used to generate stem cell-derived islet cells yield heterogenous cell populations. We generated a human embryonic stem cell line that reports insulin- and glucagon-expressing cells in vitro and in vivo without altering their differentiation or function. We showed some insulin- and glucagon-expressing bihormonal cells are cell-autonomously fated to become α-like cells. This reporter cell line can be used to further study and improve stem cell-derived islet differentiation and transplantation.
Medical subject headings
- Glucagon
- Human Embryonic Stem Cells
- Insulin
- Glucagon-Secreting Cells