Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39572737.
- Also identified by DOI 10.1038/s41587-024-02475-x.
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Abstract
Pooled single-cell CRISPR screens have profiled either gene expression or chromatin accessibility but not both modalities. Here we develop MultiPerturb-seq, a high-throughput CRISPR screening platform with joint single-nucleus chromatin accessibility, transcriptome and guide RNA capture using combinatorial indexing combined with droplet microfluidics to scale throughput and integrate all three modalities. We identify key differentiation genes in a rare pediatric cancer and establish ZNHIT1 as a potential target for cancer reprogramming therapy.
Medical subject headings
- Chromatin
- Gene Expression Profiling
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Cell Nucleus