Detecting telomerase activity at the single-cell level using a CRISPR-Cas12a-based chip.
basic_science · Level V
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- Record sourced from PubMed, PMID 39588656.
- Also identified by DOI 10.1039/d4lc00619d.
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Abstract
The intimate association between telomerase activity and cancer has driven the exploration of diverse methodologies for its precise detection. However, detecting telomerase activity at the single-cell level remains a significant challenge. Herein, we present a MOF-DNA barcode-amplified CRISPR-Cas12a strategy integrated with a single-cell microfluidic chip for ultrasensitive detection of telomerase activity. DNA-functionalized UiO-66 nanoparticles act as signal transducers, effectively converting telomerase activity into DNA activation strands, which subsequently trigger the <i>trans</i>-cleavage activity of CRISPR-Cas12a. This amplification-based assay could be integrated with a microfluidic chip to enable highly sensitive detection of telomerase activity at the single-cell level, offering promising advancements in early cancer diagnosis.
Medical subject headings
- Telomerase
- CRISPR-Cas Systems
- Single-Cell Analysis