Ultraflexible Neural Electrodes Enabled Synchronized Long-Term Dopamine Detection and Wideband Chronic Recording Deep in Brain.

Abstract

Ultraflexible neural electrodes have shown superior stability compared with rigid electrodes in long-term <i>in vivo</i> recordings, owing to their low mechanical mismatch with brain tissue. It is desirable to detect neurotransmitters as well as electrophysiological signals for months in brain science. This work proposes a stable electronic interface that can simultaneously detect neural electrical activity and dopamine concentration deep in the brain. This ultraflexible electrode is modified by a nanocomposite of reduced graphene oxide (rGO) and poly(3,4-ethylenedioxythiophene):poly(sodium 4-styrenesulfonate) (rGO/PEDOT:PSS), enhancing the electrical stability of the coating and increasing its specific surface area, thereby improving the sensitivity to dopamine response with 15 pA/μM. This electrode can detect dopamine fluctuations and can conduct long-term, stable recordings of local field potentials (LFPs), spiking activities, and amplitudes with high spatial and temporal resolution across multiple regions, especially in deep brain areas. The electrodes were implanted into the brains of rodent models to monitor the changes in neural and electrochemical signals across different brain regions during the administration of nomifensine. Ten minutes after drug injection, enhanced neuronal firing activity and increased LFP power were detected in the motor cortex and deeper cortical layers, accompanied by a gradual rise in dopamine levels with 192 ± 29 nM. The <i>in vivo</i> recording consistently demonstrates chronic high-quality neural signal monitoring with electrochemical signal stability for up to 6 weeks. These findings highlight the high quality and stability of our electrophysiological/electrochemical codetection neural electrodes, underscoring their tremendous potential for applications in neuroscience research and brain-machine interfaces.