Phosphatidylinositol 4,5-bisphosphate drives the formation of EGFR and EphA2 complexes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39630914.
- Also identified by DOI 10.1126/sciadv.adl0649 and PMC identifier 11616708.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Receptor tyrosine kinases (RTKs) regulate many cellular functions and are important targets in pharmaceutical development, particularly in cancer treatment. EGFR and EphA2 are two key RTKs that are associated with oncogenic phenotypes. Several studies have reported functional interplay between these receptors, but the mechanism of interaction is still unresolved. Here, we use a time-resolved fluorescence spectroscopy called PIE-FCCS to resolve EGFR and EphA2 interactions in live cells. We tested the role of ligands and found that EGF, but not ephrin A1 (EA1), stimulated heteromultimerization between the receptors. To determine the effect of anionic lipids, we targeted phospholipase C (PLC) activity to alter the abundance of phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>). We found that higher PIP<sub>2</sub> levels increased homomultimerization of both EGFR and EphA2, as well as heteromultimerization. This study provides a direct characterization of EGFR and EphA2 interactions in live cells and shows that PIP<sub>2</sub> can have a substantial effect on the spatial organization of RTKs.
Medical subject headings
- Receptor, EphA2
- ErbB Receptors
- Phosphatidylinositol 4,5-Diphosphate
- Protein Binding