Unraveling the bioactive constituents of Typha elephantina: A comprehensive phytochemical analysis by tandem mass spectrometry.

Abstract

Phytochemicals derived from plants have gained significant attention in recent years due to their diverse therapeutic properties. Typha elephantina is an aquatic plant having ameliorative characteristics like antioxidant, anti-inflammatory and analgesic etc. This research aims to conduct a comprehensive phytochemical investigation by Tandem mass spectrometry on the aerial parts and roots of Typha elephantina with a focus on identifying and characterizing the bioactive compounds present in it. Maceration in methanol, preliminary, MS/MS analyses and DPPH antioxidant assay were carried out on this plant. This study led to the elucidation of 62 chemical constituents for the first time in Typha elephantina. 36 phytochemical compounds from aerial parts and 26 from roots i.e.,p-coumaric acid, caffeic acid, dihydrocaffeic acid, ferulic acid derivative, dehydroascorbic acid derivative, 1-O-coumaroyl glycerol, glucaroyl-4-hydroxy benzoate, apigenin derivative, 3-O-glucopyranosyl isorhamnetin, isovitexin derivative, rutin, isorhamnetin diglycosides, verbascoside, forsythoside A, pinocembrin, dihydro quercetin, prunetin, ampelopsin, daidzein, genistein, catechin and procyanidin B1 were detected within this plant specimen. The DPPH assay results showed that aerial parts TE(1), TE(2) showed more antioxidant activity than roots TER/MeOH. These might be responsible for the understanding of the therapeutic potential of Typha elephantina and provide a foundation for future pharmacological studies.