The complete telomere-to-telomere sequence of a mouse genome.

Liu, Junli; Li, Qilin; Hu, Yixuan; Yu, Yi; Zheng, Kai; Li, Dengfeng; Qin, Lexin; Yu, Xiaochun · Science · 2024

basic_science · Level V

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Abstract

The current reference genome of <i>Mus musculus</i>, GRCm39, has major gaps in both euchromatic and heterochromatic regions associated with repetitive sequences. In this work, we have sequenced and assembled the telomere-to-telomere genome of mouse haploid embryonic stem cells. The results reveal more than 7.7% of previously uncovered sequences of the mouse genome, including ribosomal DNA arrays and pericentromeric and subtelomeric regions, as well as an additional 140 genes predicted to be protein-coding. This study helps to address knowledge gaps in the mouse genome.

Medical subject headings