Label-free single-cell RNA multiplexing leveraging genetic variability.
Where this comes from
- Record sourced from PubMed, PMID 39638798.
- Also identified by DOI 10.1038/s41467-024-54270-6 and PMC identifier 11621319.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.
Medical subject headings
- Single-Cell Analysis
- Sequence Analysis, RNA
- Genetic Variation