Neuronal Tracing and Visualization of Nerve Injury by a Membrane-Anchoring Aggregation-Induced Emission Probe.

Abstract

Deciphering neuronal circuits is pivotal for deepening our understanding of neuronal functions and advancing treatments for neurological disorders. Conventional neuronal tracers suffer from restrictions such as limited penetration depth, high immunogenicity, and inadequacy for long-term and <i>in vivo</i> imaging. In this context, we introduce an aggregation-induced emission luminogen (AIEgen), MeOTFVP, engineered for enhanced neuronal tracing and imaging. MeOTFVP is strategically designed to target cell membranes by integrating into the phospholipid bilayer through its amphipathy. The donor-acceptor molecular skeleton facilitates a red shift of its photoluminescence into the near-infrared (NIR) spectrum, significantly improving tissue penetration. The affinity of MeOTFVP for cell membranes, coupled with its deep tissue penetration, allows precise tracing in the paw-dorsal root ganglia (DRG) circuit and detailed imaging of the sciatic nerve. This study showcases the application of MeOTFVP as a dual-function neuronal tracer, propelling forward the possibilities for advanced neuronal tracing and imaging using AIEgens.