PCA-based spatial domain identification with state-of-the-art performance.

Schaub, Darius P; Yousefi, Behnam; Kaiser, Nico; Khatri, Robin; Puelles, Victor G; Krebs, Christian F; Panzer, Ulf; Bonn, Stefan · Bioinformatics · 2024

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Abstract

The identification of biologically meaningful domains is a central step in the analysis of spatial transcriptomic data. Following Occam's razor, we show that a simple PCA-based algorithm for unsupervised spatial domain identification rivals the performance of ten competing state-of-the-art methods across six single-cell spatial transcriptomic datasets. Our reductionist approach, NichePCA, provides researchers with intuitive domain interpretation and excels in execution speed, robustness, and scalability. The code is available at https://github.com/imsb-uke/nichepca.

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