Imaging of dairy emulsions <i>via</i> a novel approach of transmission electron cryogenic microscopy using beam exposure.

Daffner, Kilian; Hanssen, Eric; Norton, Ian; Mills, Tom; Ong, Lydia; Gras, Sally L · Soft Matter · 2020

basic_science · Level V

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Abstract

Emulsions containing small fat and protein particles are difficult to differentiate but low dose transmission electron cryogenic microscopy can visualize and distinguish between these droplets or particles. A controlled increase in electron dose caused the sample to bubble and visibly degrade at <25 e<sup>-</sup> Å<sup>-2</sup> for fat globules and at >150 e<sup>-</sup> Å<sup>-2</sup> for protein particles, allowing particle differentiation. This technique may be useful for the development of nanoemulsions, as well as nanostructured and 3D printed foods.