Natural variations in <i>TT8</i> and its neighboring <i>STK</i> confer yellow seed with elevated oil content in <i>Brassica juncea</i>.

Abstract

Seed color is a critical quality trait in numerous plant species. In oilseed <i>Brassica</i> crops, including rapeseed and mustard, yellow seeds are distinguished by their significantly higher oil content and faster germination rates compared to black or brown counterparts. Despite the agronomic significance of the yellow seeds being a prime breeding target, the mechanisms underlying elevated oil content remain obscure. In this study, we assembled the first telomere-to-telomere (T2T) genome of <i>B. juncea</i> and further investigated the genetic regulation, molecular mechanism, and the evolutionary history of yellow seeds in <i>B. juncea.</i> Through an analysis of allelic variation in the <i>TRANSPARENT TESTA 8</i> (<i>TT8</i>) genes across 1,002 worldwide <i>B</i>. <i>juncea</i> accessions, we traced the single origin of yellow seeds to approximately 2,300 y ago in Southwestern China. Furthermore, we discovered the MADS-box gene <i>SEEDSTICK</i> (<i>STK</i>) coevolved with <i>TT8</i>, and they coordinately regulated seed size, oil accumulation, and seed coat proportion in <i>B. juncea</i>. These findings open broad avenues for targeted breeding of yellow-seeded <i>Brassica</i> crops with elevated oil content.