Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 39905266.
- Also identified by DOI 10.1038/s41587-025-02554-7 and PMC identifier 12807852.
- Licence recorded as CC BY-NC-ND.
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Abstract
Here we report a method, smol-seq (small-molecule sequencing), using structure-switching aptamers (SSAs) and DNA sequencing to quantify metabolites. In smol-seq, each SSA detects a single target molecule and releases a unique DNA barcode on target binding. Sequencing the released barcodes can, thus, read out metabolite levels. We show that SSAs are highly specific and can be multiplexed to detect multiple targets in parallel, bringing the power of DNA sequencing to metabolomics.
Medical subject headings
- Aptamers, Nucleotide
- Sequence Analysis, DNA
- Metabolomics
- High-Throughput Nucleotide Sequencing