Pulsatile-flow culture: a novel system for assessing vascular-cell dynamics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40019369.
- Also identified by DOI 10.1039/d4lc00949e and PMC identifier 11869938.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We describe a model system for vascular-cell culture where recirculating fluid flow in standard culture plates is generated by gravity using a combination of platform tilt and rotation (nutation). Placed inside a cell-culture incubator, variable nutation speeds provide pulsatile shear stresses to vascular cells within the physiological range. The effect of these stresses on cells is demonstrated here using standard laboratory techniques such as immunofluorescent staining, immunoblot, and supernatant analyses. This gravity-driven model framework is well-suited for assessing dynamic conditions for mono- and co-cultures. In addition, the modular design and the use of off-the-shelf components make the system economical and scalable.
Medical subject headings
- Pulsatile Flow
- Cell Culture Techniques
- Microfluidic Analytical Techniques