Emerging brain organoids: 3D models to decipher, identify and revolutionize brain.

Zhao, Yuli; Wang, Ting; Liu, Jiajun; Wang, Ze; Lu, Yuan · Bioact Mater · 2025

basic_science · Level V

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Abstract

Brain organoids are an emerging <i>in vitro</i> 3D brain model that is integrated from pluripotent stem cells. This model mimics the human brain's developmental process and disease-related phenotypes to a certain extent while advancing the development of human brain-based biological intelligence. However, many limitations of brain organoid culture (e.g., lacking a functional vascular system, etc.) prevent <i>in vitro</i>-cultured organoids from truly replicating the human brain in terms of cell type and structure. To improve brain organoids' scalability, efficiency, and stability, this paper discusses important contributions of material biology and microprocessing technology in solving the related limitations of brain organoids and applying the latest imaging technology to make real-time imaging of brain organoids possible. In addition, the related applications of brain organoids, especially the development of organoid intelligence combined with artificial intelligence, are analyzed, which will help accelerate the rational design and guidance of brain organoids.