ATG6 interacting with NPR1 increases <i>Arabidopsis thaliana</i> resistance to <i>Pst</i> DC3000/<i>avrRps4</i> by increasing its nuclear accumulation and stability.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40036061.
- Also identified by DOI 10.7554/eLife.97206 and PMC identifier 11879114.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Autophagy-related gene 6 (ATG6) plays a crucial role in plant immunity. Nonexpressor of pathogenesis-related genes 1 (NPR1) acts as a signaling hub of plant immunity. However, the relationship between ATG6 and NPR1 is unclear. Here, we find that ATG6 directly interacts with NPR1. <i>ATG6</i> overexpression significantly increased nuclear accumulation of NPR1. Furthermore, we demonstrate that <i>ATG6</i> increases NPR1 protein levels and improves its stability. Interestingly, ATG6 promotes the formation of SINCs (SA-induced NPR1 condensates)-like condensates. Additionally, ATG6 and NPR1 synergistically promote the expression of <i>pathogenesis-related</i> genes. Further results showed that silencing <i>ATG6</i> in <i>NPR1-GFP</i> exacerbates <i>Pst</i> DC3000/<i>avrRps4</i> infection, while double overexpression of <i>ATG6</i> and <i>NPR1</i> synergistically inhibits <i>Pst</i> DC3000/<i>avrRps4</i> infection. In summary, our findings unveil an interplay of NPR1 with ATG6 and elucidate important molecular mechanisms for enhancing plant immunity.
Medical subject headings
- Arabidopsis
- Arabidopsis Proteins
- Plant Diseases
- Disease Resistance
- Autophagy-Related Proteins
- Pseudomonas syringae
- Cell Nucleus