Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40085503.
- Also identified by DOI 10.7554/eLife.97189 and PMC identifier 11908783.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran insect <i>Helicoverpa armigera</i> (cotton bollworm), a serious agricultural pest, as a model, we determined that receptor tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor receptor homologue (FGFR1) function as JH cell membrane receptors by their roles in JH-regulated gene expression, larval status maintaining, rapid intracellular calcium increase, phosphorylation of JH intracellular receptor MET1 and cofactor Taiman, and high affinity to JH III. Gene knockout of <i>Cad96ca</i> and <i>Fgfr1</i> by CRISPR/Cas9 in embryo and knockdown in various insect cells, and overexpression of CAD96CA and FGFR1 in mammalian HEK-293T cells all supported CAD96CA and FGFR1 transmitting JH signal as JH cell membrane receptors.
Medical subject headings
- Receptor, Fibroblast Growth Factor, Type 1
- Juvenile Hormones
- Cadherins
- Moths
- Insect Proteins
- Receptor Protein-Tyrosine Kinases