Single-Atom Cu Anchored on Carbon Nitride as a Bifunctional Glucose Oxidase and Peroxidase Nanozyme for Antibacterial Therapy.

Wu, Fan; Wang, Yaran; Li, Yuanfeng; Shi, Linqi; Yuan, Lu; Ren, Yijin; van der Mei, Henny C; Liu, Yong · ACS Nano · 2025

basic_science · Level V

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Abstract

A very promising strategy to avoid bacterial drug resistance is to replace antibiotics with artificial nanozymes, but this has not yet been translated to the clinic. Here, we construct a single-atom nanozyme using graphitic carbon nitride nanosheets modified by copper (Cu-<i>g</i>-C<sub>3</sub>N<sub>4</sub>). This Cu-<i>g</i>-C<sub>3</sub>N<sub>4</sub> nanosheet possesses both glucose oxidase-like and peroxidase-like activities responsible for reactive-oxygen-species generation by a cascade reaction to eradicate Gram-positive and Gram-negative multidrug-resistant bacteria. Cu-<i>g</i>-C<sub>3</sub>N<sub>4</sub> is introduced into polycaprolactone (PCL) by electrospinning to obtain (Cu-<i>g</i>-C<sub>3</sub>N<sub>4</sub>/PCL) nanofibers, which can be used as a dressing for bacterially infected wounds. It is demonstrated that Cu-<i>g</i>-C<sub>3</sub>N<sub>4</sub>/PCL nanofiber dressings can eradicate bacterial infections and accelerate wound healing in a mouse model with a skin wound.

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