AlphaPulldown2-a general pipeline for high-throughput structural modeling.

Molodenskiy, Dmitry; Maurer, Valentin J; Yu, Dingquan; Chojnowski, Grzegorz; Bienert, Stefan; Tauriello, Gerardo; Gilep, Konstantin; Schwede, Torsten et al. · Bioinformatics · 2025

basic_science · Level V

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Abstract

AlphaPulldown2 streamlines protein structural modeling by automating workflows, improving code adaptability, and optimizing data management for large-scale applications. It introduces an automated Snakemake pipeline, compressed data storage, support for additional modeling backends like UniFold and AlphaLink2, and a range of other improvements. These upgrades make AlphaPulldown2 a versatile platform for predicting both binary interactions and complex multi-unit assemblies. AlphaPulldown2 is freely available at https://github.com/KosinskiLab/AlphaPulldown.

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