Prediction of impedance characteristic during electrical stimulation with microelectrode arrays.

Abstract

<i>Objective.</i>Modern neural devices allow to interact with degenerated tissue in order to restore sensoric loss function and to suppress symptoms of neurodegenerative diseases using microelectronic arrays (MEA). They have a bidirectional interface for performing electrical stimulation to write-in new information and for recording the neural activity to read-out a neural task, e.g. movement ambitions. For both applications, the electrical impedance of the electrode-tissue interface (ETI) is crucial. However, the ETI can change during run-time due to encapsulation effects and changes of the neuronal structures. We investigated if an impedance spectrum can be reliably extracted from recordings during stimulation with microelectrode arrays.<i>Approach.</i>We present a measurement method for characterizing the electrical impedance spectrum during stimulation. We performed charge-controlled stimulation with a penetrating microelectrode array in an electrolyte solution. From the stimulation recordings, we extracted the impedance. Furthermore, a numerical model (digital twin) of the stimulation electrodes is established.<i>Main results.</i>We obtained consistent results for relevant electrochemical using electrochemical impedance spectroscopy, time-domain analysis and Fourier-transform-based impedance estimation. Moreover, the numerical simulations confirmed that the measured microelectrode had the expected properties.<i>Significance.</i>Our results pave the way to enable a live assessment of the impedance in future MEA-based neural devices. This will enable adaptive electrical stimulation or (re-)selection of recording electrodes by taking the actual state of the electrode into account.