NanoASV: a snakemake workflow for reproducible field-based Nanopore full-length 16S metabarcoding amplicon data analysis.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 40111866.
- Also identified by DOI 10.1093/bioinformatics/btaf089 and PMC identifier 11937976.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
NanoASV is a conda environment and snakemake-based workflow using state-of-the-art bioinformatics software to process full-length SSU rRNA (16S/18S) amplicons acquired with Oxford Nanopore Sequencing technology. Its strength lies in reproducibility, portability, and the possibility to run offline, allowing in-field analysis. It can be installed on the Nanopore MK1C sequencing device and process data locally. Source code and documentation are freely available at https://github.com/ImagoXV/NanoASV and Zenodo archive at https://doi.org/10.5281/zenodo.14730742.
Medical subject headings
- Software
- RNA, Ribosomal, 16S
- Nanopore Sequencing
- Nanopores
- Computational Biology
- DNA Barcoding, Taxonomic