AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA.
basic_science · Level V
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- Record sourced from PubMed, PMID 40178896.
- Also identified by DOI 10.1073/pnas.2423205122 and PMC identifier 12002350.
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Abstract
The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition.
Medical subject headings
- DNA, Single-Stranded
- CRISPR-Cas Systems
- Bacterial Proteins
- CRISPR-Associated Proteins