Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40191992.
- Also identified by DOI 10.1021/acs.nanolett.5c00877 and PMC identifier 12007104.
- Licence recorded as CC BY-NC-ND.
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Abstract
Protein function investigation and clinical assay are fundamental to modern biology and medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication and repair, plays a critical role in the progression of many diseases. Taking FEN1 as an example, we present a novel protein detection platform combining triboelectric nanogenerator (TENG) and CRISPR/Cas technologies. As a specific form of TENG, the transistor-droplet electricity generator (TDEG) is explored, which offers a low-cost, simple fabrication approach with real-time detection capability. Meanwhile, the FEN1 activated CRISPR/Cas system catalyzes the reactions on the three-dimensional DNA tetrahedron interface, promising the high sensitivity. This work not only demonstrates a powerful method for rapid protein detection but also pioneers the integration of CRISPR/Cas with TENG. It has a great prospect for future development of TENG sensors.
Medical subject headings
- CRISPR-Cas Systems
- Polymers
- Indoles
- Flap Endonucleases
- Biosensing Techniques