Far-red fluorescent genetically encoded calcium ion indicators.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40195305.
- Also identified by DOI 10.1038/s41467-025-58485-z and PMC identifier 11977202.
- Licence recorded as CC BY-NC-ND.
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Abstract
Genetically encoded calcium ion (Ca<sup>2+</sup>) indicators (GECIs) are widely-used molecular tools for functional imaging of Ca<sup>2+</sup> dynamics and neuronal activities with single-cell resolution. Here we report the design and development of two far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, based on the monomeric far-red fluorescent proteins mKelly1 and mKelly2. FR-GECOs have excitation and emission maxima at ~596 nm and ~644 nm, respectively, display large responses to Ca<sup>2+</sup> in vitro (ΔF/F<sub>0</sub> = 6 for FR-GECO1a, 18 for FR-GECO1c), are bright under both one-photon and two-photon illumination, and have high affinities (apparent K<sub>d</sub> = 29 nM for FR-GECO1a, 83 nM for FR-GECO1c) for Ca<sup>2+</sup>. FR-GECOs offer sensitive and fast detection of single action potentials in neurons, and enable in vivo all-optical manipulation and measurement of cellular activities in combination with optogenetic actuators.
Medical subject headings
- Calcium
- Luminescent Proteins