Limitations and optimizations of cellular lineages tracking.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40228207.
- Also identified by DOI 10.1371/journal.pcbi.1012880 and PMC identifier 11996212.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Tracking cellular lineages using genetic barcodes provides insights across biology and has become an important tool. However, barcoding strategies remain ad hoc. We show that elevating barcode insertion probability and thus increasing the average number of barcodes within the cells, adds to the number of traceable lineages but may decrease the accuracy of lineages inference due to reading errors. We establish the trade-off between accuracy in tracing lineages and the total number of traceable lineages, and find optimal experimental parameters under limited resources concerning the populations size of tracked cells and barcode pool complexity.
Medical subject headings
- Cell Lineage
- DNA Barcoding, Taxonomic
- Cell Tracking