Unified molecular approach for spatial epigenome, transcriptome, and cell lineages.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40249782.
- Also identified by DOI 10.1073/pnas.2424070122 and PMC identifier 12037033.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Spatial epigenomics and multiomics can provide fine-grained insights into cellular states but their widespread adoption is limited by the requirement for bespoke slides and capture chemistries for each data modality. Here, we present SPatial assay for Accessible chromatin, Cell lineages, and gene Expression with sequencing (SPACE-seq), a method that utilizes polyadenine-tailed epigenomic libraries to enable facile spatial multiomics using standard whole transcriptome reagents. Applying SPACE-seq to a human glioblastoma specimen, we reveal the state of the tumor microenvironment, extrachromosomal DNA copy numbers, and identify putative mitochondrial DNA variants.
Medical subject headings
- Transcriptome
- Epigenome
- Cell Lineage
- Epigenomics
- Glioblastoma